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KMID : 1137020080190020139
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Journal of Gynecologic Oncology
2008 Volume.19 No. 2 p.139 ~ p.144
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Original Article : Tenascin-X and Leukemia inhibitory factor receptor are down-regulated in Leiomyoma compared with normal myometrium
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Lee Sun- Ok
Lee Soo-Yoon
Lee Sa-Ra
Ju Woong
Kim Seung-Cheol
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Abstract
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Objectives: Uterine leiomyomas are the most common tumor of the uterus. But the molecular causes of uterine
leiomyoma remain unclear. We conducted the current investigation in order to elucidate the molecular mechanisms in
the development of uterine leiomyoma.
Methods: We employed a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) method that involved annealing control primers (ACPs) to identify the genes that are differently expressed in uterine leiomyoma.
Results: Using 120 ACPs, we identified and sequenced 14 differently expressed genes (DEGs) in uterine leiomyoma compared with normal myometrium. Basic Local Alignment Search Tool (BLAST) searches were performed to
examine the known functions of these genes associated with uterine leiomyoma. We confirmed differently expressed
patterns in more cases using the RT-PCR method. We also detected two novel genes, Tenascin-X and Leukemia Inhibitory Factor Receptor (LIFR), which had not yet been reported to have any functions associated with uterine
leiomyoma. RT-PCR confirmation shows that both of these two genes are down-regulated in uterine leiomyoma.
Conclusion: Our results suggest that Tenascin-X and LIFR may play a role in the development of uterine leiomyoma. Although further studies are required to establish the precise mechanisms with which these genes are involved in the genesis of uterine leiomyoma, the present research is significant in that it is the first study which detects
down-regulated novel genes in uterine leiomyoma using the ACP system.
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KEYWORD
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Uterine Leiomyoma, Tenascin-X, Leukemia inhibitory factor receptor, LIFR, Annealing control primers, ACP, system
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